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Research Library

Protocol Library

Published research protocols for reference use. Each entry cites primary literature. Not prescriptive — always follow your institution's protocols and ethical guidelines.

13 protocols found
BPC-157In vivo · Rodent

Subcutaneous Administration — Angiogenesis & Tissue Repair Model

Lyophilized BPC-157 dissolved in sterile saline. Administered daily for 14 days in a Wistar rat model of tendon transection. Endpoint: histological assessment of collagen organization and vascular density.

Route
Subcutaneous / IP
Dose
10–15 mcg/kg
Duration
14 days
Frequency
Once daily
Sikiric et al. (2020) Gwyer et al. (2019)
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BPC-157In vivo · Rodent

Intragastric Administration — GI Mucosa Model

BPC-157 administered in drinking water (diluted) or via gavage. Model: ethanol-induced gastric ulceration in Sprague-Dawley rats. Endpoint: macroscopic ulcer score, COX-2/iNOS expression.

Route
Oral / Intragastric
Dose
10 mcg/kg
Duration
7 days
Frequency
Once daily
Sikiric et al. (2018)
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TB-500In vivo · Rodent

Systemic Administration — Full-Thickness Wound Healing Model

Thymosin β4 (Tβ4) administered IP in a full-thickness excisional wound model in C57BL/6 mice. Endpoints: wound closure rate, CD31 staining for angiogenesis, mRNA expression of keratinocyte migration markers.

Route
Intraperitoneal (IP)
Dose
25 mg/kg
Duration
7 days
Frequency
Day 1 and Day 4
Goldstein et al. (2012) Sosne et al. (2010)
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TB-500In vivo · Rodent

IP Administration — Cardiac Progenitor Cell Activation Model

Tβ4 administered post-MI in a murine ligation model. Endpoint: echocardiographic function, Ki67 positivity in cardiomyocytes, EPCs mobilized from bone marrow.

Route
Intraperitoneal (IP)
Dose
150 mcg/kg
Duration
8 weeks
Frequency
Twice weekly
Smart et al. (2007)
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GHK-CuIn vitro · Human cell line

In Vitro Cell Culture — Dermal Fibroblast Collagen Synthesis

GHK-Cu dissolved in PBS and added to human dermal fibroblast (HDF) culture. Endpoint: RT-PCR for COL1A1/COL3A1, MMP-1/-2 activity assay, wound-scratch migration assay. Optimal response observed at 1–100 nM.

Route
Culture medium (dissolved)
Dose
1 nM – 1 µM
Duration
48–72 h
Frequency
Single treatment
Pickart et al. (2015) Gorouhi & Maibach (2009)
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GHK-CuIn vivo · Rodent

Subcutaneous Administration — Dermal Repair In Vivo

GHK-Cu injected subcutaneously adjacent to incisional wound in Sprague-Dawley rats. Endpoint: breaking strength of healed tissue, histological collagen density score.

Route
Subcutaneous
Dose
1–2 mg/kg
Duration
10 days
Frequency
Once daily
Pickart & Margolina (2018)
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NAD+In vivo · Rodent

IP Administration — Mitochondrial Function & NAD+/NADH Ratio

NAD+ administered IP to aging C57BL/6 mice. Endpoints: hepatic and skeletal muscle NAD+/NADH ratio (HPLC), SIRT1 and SIRT3 protein expression (Western blot), mtDNA copy number.

Route
Intraperitoneal (IP)
Dose
250–500 mg/kg
Duration
7 days
Frequency
Once daily
Rajman et al. (2018) Mills et al. (2016)
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SemaxIn vivo · Rodent

Intranasal Administration — BDNF & NGF Upregulation Model

Semax administered intranasally in Wistar rats. Endpoints: hippocampal BDNF, NGF mRNA quantification via RT-PCR; Morris water maze performance; Fos protein expression in prefrontal cortex.

Route
Intranasal
Dose
50–200 mcg/kg
Duration
5 days
Frequency
Once daily
Dolotov et al. (2006) Agapova et al. (2007)
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SelankIn vivo · Rodent

IP Administration — Anxiolytic Activity in EPM Model

Selank administered IP 30 minutes prior to elevated-plus-maze (EPM) testing in Wistar rats. Endpoint: open-arm time percentage, head-dipping frequency, locomotion index. Effective at 0.3 mg/kg.

Route
Intraperitoneal (IP)
Dose
0.3–3 mg/kg
Duration
Acute
Frequency
Single injection, 30 min pre-test
Semenova et al. (2010)
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EpithalonIn vivo · Rodent + Cell culture

IP Administration — Telomere Length & Telomerase Activity

Epithalon (Ala-Glu-Asp-Gly tetrapeptide) administered IP in aging Wistar rats. Endpoints: telomere length via Q-FISH, TRAP assay for telomerase activity, mean and max lifespan in long-term cohort.

Route
Intraperitoneal (IP)
Dose
0.1–3 mg/kg
Duration
3 weeks
Frequency
Once daily, 5 days on / 2 days off
Anisimov et al. (2003) Khavinson et al. (2004)
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MOTS-cIn vivo · Rodent

IP Administration — Insulin Sensitivity & Metabolic Regulation

MOTS-c (a mitochondria-derived peptide) administered IP in high-fat-diet C57BL/6 mice. Endpoints: glucose tolerance test (GTT), insulin tolerance test (ITT), AMPK phosphorylation in skeletal muscle, body weight.

Route
Intraperitoneal (IP)
Dose
5 mg/kg
Duration
2 weeks
Frequency
Once daily
Lee et al. (2015)
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SS-31In vivo · Rodent

IV / IP Administration — Mitochondrial Oxidative Stress Model

SS-31 (Elamipretide) targets the inner mitochondrial membrane. Administered IV or IP in models of ischemia-reperfusion injury. Endpoints: mtROS (MitoSOX), Δψm (JC-1), ATP production, histological infarct area.

Route
IV or IP
Dose
1–5 mg/kg
Duration
14 days
Frequency
Once daily
Szeto (2014) Dai et al. (2013)
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KPVIn vivo · Rodent

IP / Topical Administration — Inflammatory Bowel Model

KPV (Lys-Pro-Val), the C-terminal fragment of alpha-MSH, administered in a DSS-induced colitis model. Endpoints: colon length, MPO activity, TNF-α/IL-6 levels (ELISA), histological inflammation score.

Route
IP / Oral / Topical
Dose
0.1–1 mg/kg
Duration
7 days
Frequency
Once daily
Dalmasso et al. (2008)
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Disclaimer. All protocols are derived from published academic literature and are provided for reference purposes only. Helix Research does not endorse any specific protocol. All compounds are supplied for laboratory research use only and are not intended for human use. Comply with all applicable laws and your institution's ethical review requirements.